Ftir spectroscopy for grape and wine analysis analytical. Research article using ftir, hplc and gcms analysis. Journal of food composition and analysis 2011, 24 45, 548562. This means that, in principle, reflectance ftirm, which is nondestructive, can be used to obtain spectra that are directly comparable not only with transmission ftirm spectra but also with the more traditional kbr pellet transmission spectra. Ftir spectroscopy offers a vast array of analytical opportunities in academic, analytical, qaqc and forensic labs. Infrared ir spectroscopy combined with chemometrics has been developed for simple analysis of flavonoid in the medicinal plant extract. Activity and phytochemical analysis of indian medicinal plant couroupita guianensis aubl, journal of chemical and pharmaceutical research 36 2011,115121 6 b. To evaluate the amount of flavonoids present in the drugs extracts, 0. Techniques for the analysis of pentacyclic triterpenoids in. Clitoria ternatea, methanolic extract, phytochemical screening, ftir spectra. Phytochemical screening by ftir spectroscopic analysis of. Analysis of flavonoid in medicinal plant extract using infrared spectroscopy and chemometrics article pdf available in journal of analytical methods in chemistry 201612. In developing nations, more than 80% of the people depend on traditional medicines for health care. Ftir analysis and quantification of phenols and flavonoids of.
Tinospora cordifolia is one of the most important medicinal plants and used in formation of important drug in ayurvedic system of medicine. The analysis methods include gas chromatography, liquid chromatography, capillary electrophoresis, thin. Research of indonesian medicinal plants using modern laboratory facilities has been started since 1970, but only about 200 plants were studied. This technique presents a rapid, inexpensive, and rather noninvasive method for obtaining chemical characteristics of a biological sample. Pdf analysis of flavonoid in medicinal plant extract. Flavonoid was extracted from medicinal plant leaves by ultrasonication and maceration. Distribution of medicinal plants b the distribution analysis of the medicinal plants shows that they are distributed across diverse habitats and landscape elements. Phytochemical analysis, antibacterial activity, ftir and. Phytochemical screening and ftir analysis of clitoria ternatea leaves. Dried powders of different solvent extracts of each plant material were used for ftir analysis. Effect of plant sample preparation and measuring methods. Phytochemical screening and gcms, ftir analysis of methanolic extract leaves of.
The most important of these bioactive constituents of plants are alkaloids, tannins, flavonoids, and phenolic compounds 1. Pdf ftir in medicinal plants komal kumar j academia. Increasing role of the who monographs on selected medicinal plants over the past two decades, there has been a tremendous increase in the use of herbal medicine. Ftir spectroscopy is proved to be a sophisticated instrument to analyse the components of the plant cells. Identification of the chemical nature of phytochemical compounds present in the medicinal plants will provide some information on the different functional groups responsible for their medicinal. It is prescribed for many diseases such as fever, general debility, diabetes, urinary infections, jaundice and skin diseases. On the nature of vibrational bands in the ftir spectra of medicinal scholars research. Ftir studies confirm the presence of biomolecules and metal oxides. Ftir analysis of the methanol extract given the major. Quick and realtime potency determination of cannabinoids. Preliminary phytochemical screening of six medicinal plants used in traditional medicine manjulika yadav, sanjukta chatterji, sharad kumar gupta and geeta watal alternative therapeutics unit, drug development division, medicinal research lab, department of chemistry, university of allahabad, allahabad211 002, u.
Analysis of curcumin in curcuma longa and curcuma xanthorriza using ftir spectroscopy and chemometrics. Phytochemical analysis, antibacterial activity, ftir and gcms. Ftir analysis the ftir spectra of plant parts like leaves, stems and seeds of selected medicinal plants are shown in figures 9 14. Developing ftir microspectroscopy for analysis of plant. Abdul rohman, sudjadi, devi, dwiky ramadhani and ardi nugroho, 2015. Ftir and gcms analysis of bioactive phytocompounds in. Isolation and characterisation of medicinal compounds from.
These authors generated absorbance spectra for a range of residue types including bone, muscle, and adipose fat using ftir atr attenuated total reflectance spectroscopy. Analysis of flavonoid in medicinal plant extract using infrared spectroscopy and chemometrics article pdf available in journal of analytical methods in. Previously optimized methods can be used to lead in the selection of suitable methods. Medicinal plants have had a crucial role in human culture and civilization. Ftir analysis and uvvis analysis showed the presence of flavonoids and a phenolic compound. The rhizomes of the plant nervilia aragoana were collected, washed, shade dried and powdered. Isolation and characterization of bioactive compounds from. Ftir spectroscopic analysis fourier transform infrared spectrophotometer ftir is perhaps the most powerful tools for identifying the types of chemical bonds functional groups present in compounds. Functional group analysis of moringa concanensis nimmo moringaceae by ftir spectrum 31. Research of indonesian medicinal plants using modern laboratory facilities has been started since, but only aboutplantswerestudied.
Jul 16, 2018 cassia tora is a plant of medicinal importance. Nov 24, 2016 ftir is an inexpensive and accurate method that provides an excellent method for monitoring thc and cbd levels in realtime 6,7. The use of traditional medicine and medicinal plants in most developing countries, as a normative basis for the maintenance of good health, has been widely observed and about 80% of the worlds population relies on herbal medicines 2. Deeply ingrained in everything from simple compound identification to process and regulatory monitoring, ftir covers a wide range of chemical applications, especially for polymers and organic compounds. Ftir analysis was done by the instrument ftir6300 type, s. Ethanol extract ether extract and methanol extract from the marc of ether extract were prepared by simple maceration process and soxhalation method. The potential of ftir analysis greatly exceeds this use, however. Ftir and gcms spectroscopic analysis of methanol and. Unique chemical signatures from each compound are then used to develop the multivariate calibration model to calculate the potency value. Xray diffraction xrd structural analysis reveals the formation of pure hexagonal phase structures of zno nanoparticles.
Ftir analysis for five selected green leafy vegetables glvs viz. Noa021261024 bmdlabs company in standard light sources by using tgs detector at resolution 4cm1. In this study chloroform extract of cassia fistula leaves was screened and characterized by ftir, gcms and hplc for dpph free. Ftir studies and spectrophotometric analysis of natural. Ir spectra of selected medicinal plant extract were correlated with flavonoid content using chemometrics. Analysis of flavonoid in medicinal plant extract using. Nigeria is endowed with a lot of medicinal plants used as alternate medicine for diseases of man. Phytochemical screening and ftir analysis of ficus. The equipment used was perkin elmer spectrum gx, 16 scans per samples in the region of 4000650 cm 1. Phytochemical analysis of hybanthus enneaspermus using.
Specac provide a range of atr accessories that can be used with a wide range of spectrometers in the analysis of solid samples such as cannabis flowers. Medicinal plants from different localities are believed to differ in their therapeutic potency. Effect of plant sample preparation and measuring methods on. A survey of literature revealed that ftir and uvvis analysis of this medicinal plant was not done so far, thus, the present work has been aimed to produce uvvis and ftir profiles of leaf extract to identify the phytoconstituents. Phytochemical analysis of hybanthus enneaspermus using uv. The history of herbal medicine is almost as old as human civilization, they are in great demand because of their great efficacy in treatment of. Ftir spectroscopy was used to identify the cellular components of the medicinal plants of mimosa pudica and caesalpinia pulcherrima. Josephs college autonomous, tiruchirappalli 620 002, tamil nadu, india 2centre for plant biotechnology, department of plant biology and plant biotechnology, st. Antibacterial and phytochemical analysis of piper nigrum.
The ftir analysis was used to identify the capping, reducing and stabilizing capacity of the leaf extract. Ftir analysis and quantification of phenols and flavonoids. Medicinal plants are in use for thousands of years and are renowned for their effectiveness in various treatments2. Population authentication of the traditional medicinal. Therefore, the analysis of chemical constituents would help in determining various biological. Qualitative and quantitative phytochemical analyses of alcoholic and aquatic crude extracts of leaves of selected nonflowering indoor plants were assessed using standard protocols and later compared with ftir analyses. A large number of medicinal plants are used as alternate medicine for diseases of man and other animals since most of them are without side effects when compared with synthetic drugs. Phytochemical analysis of stylosanthes fruticosa using uv. Review article general overview of medicinal plants. Fourier transform infrared spectroscopy ftir analysis. Jun 14, 2019 cannabis remained present in all pharmacies as a medicinal plant with numerous uses at the. Ftir and energy dispersive xray analysis of medicinal. Onsite cannabis analysis using infrared spectroscopy. The leaf extract obtained at the flask finally collected after the completion of soxhlation.
One of the plants, that deserve attention is because of its multipotent bioactive clitoria ternatea. Many of these indigenous medicinal plants are used as spices and food plants. The chemometric method used for calibration analysis was partial. It is highly valuable medicinal plant with carminative, antibacterial, antibiotic, hypoglycemic and anti fertility properties ftir fourier transform infrared spectroscopy has made a remarkable role in the field of medicinal plant analysis. Fourier transform infrared ftir spectroscopic analysis of. The ftir findings indicated the presence of characteristic functional groups of alkanes, alkenes, alkynes, alcohols, amides, amines, carboxylic acid. The medicinal value of these plants lies in some chemical substances that produce a definite physiological action on the human body. Medicinal plants are abundantly available all over the world and are more focused than ever because they have the ability to produce many benefits to human society, especially for the treatment of various types of human ailments. Identification of the chemical nature of phytochemical compounds present in the medicinal plants will provide some information on the different functional groups responsible for their medicinal properties.
Phytochemical and ftir analysis of tinospora cordifolia. Ftir analysis the ftir measurement of plant extract and copper nanoparticles are shown in fig 2a and2b, respectively. Pdf analysis of flavonoid in medicinal plant extract using. Plants have great potential uses especially traditional, pharmacopoeial drugs. The ftir spectra had amply evidenced the occurrence of oh group together with the terpenoids, and phenol.
Functional group analysis of moringa concanensis nimmo moringaceae by ftir spectrum 29 ii. The fouriertransform infrared spectroscopy ftir analysis of the drugs was performed on a drop of each drug. Fourier transform infrared spectroscopy analysis of few. With the help of appropriate reference samples, a calibration could be created, allowing quantitative cannabis analysis i. Therefore, the effects of 2 measurement methods were investigated. This figure shows a very small portion of the overall number of medicinal plant species that were reported. Phytochemical screening and ftir analysis of clitoria. Ftir and gcms spectroscopic analysis of methanol and chloroform extracts of brenania brieyi root bark. Functional group analysis of moringa concanensis nimmo. Research article analysis of flavonoid in medicinal plant. Elemental analysis for categorization of wines and authentication of their certified brand of origin. Ftir spectroscopic analysis of methanolic leaf extract of ampelocissus latifolia for antimicrobial compounds.
Ftir analysis of cannabis, due to the wide range of cannabinoids present, could be problematic because peaks resulting from thc could be masked by other compounds. Ftir can be used to monitor how plant potency changes over time, as the plant grows, and how any changes in the external environment have effected thc and cbd production. Ftir spectroscopy allows a reliable distinction between thcs and cbds hemp within a few seconds. The ftir analysis of leaf powder of the plant revealed the following functional groups in which the frequency ranges are 36003200 cm1, 30002850 cm1,17401720 cm1, 16801620 cm1,1400 cm1, 20 cm1, 12501080 cm1, 910665 cm1. Ftir spectroscopy has been used to analyze complex spectra and matrices, a good definition of cannabinoids, using chemometric methodology. The medicinal plants are of great importance to the different types of human diseases without side effects 1. The chemometric method used for calibration analysis. Plants are having natural antioxidants like phytochemicals such as phenols, flavonoids etc capable to scavenging harmful free radicals15. Ftir spectroscopy basics thermo fisher scientific tr. A survey of literature revealed that the ftir analysis of functional groups was not done so far with the medicinal plants such as phyllanthus amarus, senna auriculata phyllanthus maderaspatensis and solanum torvum. Extraction is the crucial first step in the analysis of medicinal plants, because it is necessary to extract the desired chemical components from the plant materials for further separation and characterization. The measurements made by ftir were extremely accurate and reproducible. Introduction medicinal plant research includes much more than the discovery of new drugs.
Medicinal plants have served as a constant source of medicaments, which have a great efficacy and demand for the treatment of various diseases. However, the possibilities of ftir analysis go far beyond that. Spectroscopic uvvis, ftir methods together or separate can be used in this sense as well as conventional methods ibrahim et al. Plants derived natural products such alkaloids, flavonoids, terpenoids have received considerable. Ftir and energy dispersive xray analysis of medicinal plants. Nov 20, 2017 ftir analysis the chemical compositions of plant extract, quercetin and the synthesized silver nanoparticles were studied using ftir spectrometer thermo scientific nicolet 380. Green synthesis and characterization of zinc oxide. The most methodologically detailed publication on the application of ftirm to residues on stone tools, to date, is that of prinsloo et al.
Nearly about 70% of the medicinal plants in india are found in tropical forests in eastern and western ghats, chota nagpur plateau, aravalis. International journal of health sciences and research. Fouriertransform infrared ftir spectroscopy is an effective tool for chemical analysis of biological material berthomieu and hienerwadel, 2009. American journal of research communication, 2017, 53. Atr ftir spectra depend not only on sample preparation methods, but also on the measurement method.
The powdered sample of each plant specimen was loaded in ftir spectroscope shimadzu, ir affinity1, japan, with a scan range from 400 to 4000 cm 1 with a resolution of 4cm 1. Phytochemical screening and gcms, ftir analysis of. This methanol extract was taken to the ftir and gcms analysis. The equipment used was perkin elmer spectrum gx, 16 scans per samples in the region of 4000650 cm1. Population authentication of the traditional medicinal plant. The biomolecules present in plant extract responsible for the reduction of silver ions to agnps were identified using ftir analysis.
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